Suited for drug development, assay validation, cell therapy tissue engineering, and other immunology uses
Excess of 90% purity
Purified from mononuclear cells through immunomagnetic separation
Positive and negative selections available
All cell products are supplied with relevant documentation, including Certificate of Analysis (CoA) and thawing instructions
Cryopreserved samples with an average of over 90% viability and recovery when thawed
Request donors by HLA typing, demographics, specific disease state and more
Rapid shipping available
Cell Generation provides human CD14 monocytes purified from human peripheral blood mononuclear cell through immunomagnetic separation for a variety of research and development applications from immunology to cancer therapy. Cytometric analysis ensures that samples are successfully purified for CD14 monocytes, to meet the necessary downstream requirements.
Our mononuclear cells come from Institutional Review Board (IRB) consented donors, in accordance with all health, safety, and privacy protocols. We collect important demographic and health data to ensure that all samples carry a comprehensive assay.
Cell Generation relies on an extensive, ethnically diverse network of healthy donors for our human CD14 monocytes. These donors are thoroughly screened to ensure they are not positive for a comprehensive suite of infectious diseases and determine eligibility by a medical doctor. All donors are Institutional Review Board (IRB) approved consented donors. For each collection a detailed Certificate of Analysis (CoA) is supplied to bring the most comprehensive data set for any research application. Specific donor request is available.
Our team takes every precaution to ensure that our customers receive a viable and high-quality CD14 monocytes in every order. All processes, from donor screening to cryopreservation, follow current Good Manufacturing Practice (GMP) guidelines in order to ensure donor safety, and product high standard reproducibility. All lots undergo routine sampling and testing to guarantee our stringent quality standards are met. These samples undergo a full suite of testing for infectious diseases, fungi, mycoplasma, and more. Additional methods for determining cell count to ensure accuracy are used. A Certificate of Analysis (CoA) accompanies every shipment, showing the results of our extensive quality testing.
To ensure preservation while maintaining high viability and recovery, Cell Generation utilizes the latest cryopreservation methodology and techniques. Our cryopreservation protocols deliver high quality products while preventing contamination. These techniques gradually reduce the temperature of the CD14 monocytes below -135 °C to allow for perfect preservation with average recovery and viability in excess of 90%. All cryopreserved products are supplied with relevant documentation and thawing instructions.
Available In:
10 X 106 ≤ cells per vial
20 X 106 ≤ cells per vial
40 X 106 ≤ cells per vial
Custom orders available
Separation method: Immunomagnetic, negative selection available
Species: Human
Sample Format: Cryopreserved
Purity: over 90%
Viabilty and recovery when thawed: Upwards of 85%
Our CD14 monocytes are enriched from peripheral blood mononuclear cells that have been collected through leukapheresis separation. From these concentrated samples, our team isolates the PBMCs subpopulation, CD14 monocytes using immunomagnetic beads. The isolated purified CD14 monocytes are cryopreserved and tested through flow cytometry to determine purity and product quality. These procedures are carried out in carefully sterilized environments, under our thorough Standard Operating Procedures (SOPs) for quality control and reproducibility.
Negative immunomagnetic separation is available upon request.
CD14 monocytes serve as an essential tool for research into the complex interplay of monocytes, macrophages, and dendritic cells. They are an essential research asset in the areas of:
- Myeloid cell research
- Cell therapy drug development
- Macrophage generation
- Dendritic cell differentiation
- General immunology applications
For your unique research requirements, we can provide donor requests as needed. Reach out to our team today with the requirements for your experiment, and we can help source the CD14 monocytes you need, including requests for:
- Specific HLA typing, Class I and Class II alleles
- Specific demographics, sex, and ethnicity
- Specific health categories like BMI and smoking status
- Specific disease state
Cell Generation maintains a strong commitment to ensuring the ethical collection and use of all donor samples. We source our cells from consenting volunteer donors using Institutional Review Board (IRB) approved consent practices. Donors sign a consent form before taking part in the donation program, and industry best practice confidentiality measures are in place to ensure the privacy of all donors.
- Professional Experience
Our professional experts carry out all quality control and preservation activities with the benefit of years of experience and training in the latest methodologies. This ensures you get the highest quality and most reliable products. - We Work With You
Our team is ready to support your research endeavors in any way we can. From assay design to data analysis, our scientists can ensure you’re getting the accurate results you need with our reliable and well-documented samples. - Making Things Simple
We ensure that you’re not wasting precious research time on securing samples. With an extensive inventory, fast delivery, and responsive service, we can let your team focus on their research instead of sample procurement. - A Personal Touch
We understand how difficult the research landscape can be. That’s why we strive to provide the most reliable service for all of our valued customers. Reach out to us with any of your special requests, and we’ll do everything we can to find a suitable solution.
Get a quote